Independent bioinformatics analyst

RNA-seq and splicing analysis support for research teams.

I turn transcriptomics data into clear tables, publication-ready figures, reproducible scripts, and short reports. The work is research support, not clinical diagnosis or patient-level interpretation.

  • RNA-seq differential expression workflows with count filtering, QC summaries, DEG tables, PCA, heatmaps, and volcano plots
  • Alternative splicing support focused on rMATS outputs, candidate event review, and sashimi-ready handoff
  • GSEA/pathway interpretation that turns gene lists into readable research notes
  • Exploratory metabolic-modeling demos with Human-GEM (iMAT, GIMME)
RNA-seq workflow output GitHub portfolio
PCA Heatmap Volcano DEG table
QC summary Result tables Methods note
749 DEGsfrom public airway RNA-seq, with the full report 785 splicing eventsfiltered candidates from an exploratory rMATS analysis 13,070 reactionsmodeled per sample in a synthetic iMAT demo

Research-ready inputs

Count matrices, quantification outputs, metadata, contrasts, and authorized public/anonymized datasets.

Established open-source tools

R, Python, DESeq2-style analysis, rMATS splicing review, GSEA/pathway summaries.

Publication-ready handoff

Figures, result tables, scripts, and concise methods notes organized for researcher review.

For researchers who need analysis outputs they can inspect, explain, and reuse.

The work stays focused: transcriptomics, alternative splicing, scientific visualization, pathway summaries, and small automation tools for wet-lab data handling.

What I offer

Splicing

Alternative Splicing Review

rMATS workflow notes, exon skipping and junction-level summaries, event filtering, and sashimi plot preparation for selected genes.

Figures

Publication-Ready Visuals

PCA, sample-distance heatmaps, DEG heatmaps, volcano plots, pathway plots, expression summaries, and clean figure exports for manuscript workflows.

Pathways

Functional Interpretation

GSEA and enrichment-oriented summaries that translate ranked gene lists or DEG sets into readable pathway-level research notes.

Also available: small Python/Bash helpers for repetitive lab data tasks.

See services and packages

Selected work

R / DESeq2

RNA-seq Workflow

End-to-end DEG workflow with count data, metadata, QC, DESeq2 analysis, PCA, heatmaps, volcano plot, result tables, and a short interpretation report.

DEG table PCA Heatmap Volcano Report
Open repository
STAR / rMATS

Alternative Splicing Notes

A public rMATS skipped-exon analysis example with event filtering and candidate review notes.

Open repository
Python / Human-GEM

iMAT Tool

Interactive terminal tool for per-sample Human-GEM iMAT, Fisher and reporter-like analysis, and optional gene zeroing, with a synthetic demo.

Open project

See all work

Ready to discuss a dataset?

Send organism, data type, sample groups, contrast, and expected outputs, and say whether you need figures, tables, scripts, or a short report. Initial projects can be coordinated through trusted freelance platforms or a structured project brief.

burakkeskinin@gmail.com

I usually reply within 24-48 hours.